TABLE 2.

Gene, Protein name and Function are derived from https://www.uniprot.org.

Gene	Protein name	Function
Lysosomes related genes
LAMP1	Lysosome-associated membrane glycoprotein 1	It presents carbohydrate ligands to selectins.
LAMP2	Lysosome-associated membrane glycoprotein 2	It plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-life.
CTSD	Cathepsin D	Acid protease active in intracellular protein breakdown.
NPC1	NPC intracellular cholesterol transporter 1	Intracellular cholesterol transporter that acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment.
ATP6V1B2	V-type proton ATPase subunit B	Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
TFEB	Transcription factor EB	It plays a central role in the expression of lysosomal genes. It acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy.
Autophagy related gene
MTOR	Serine/threonine-protein kinase mTOR	Serine/threonine protein kinase playing a central regulatory role in cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals.
ULK1	Serine/threonine-protein kinase ULK1	Serine/threonine-protein kinase involved in autophagy in response to starvation. It acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes.
BECN1	Beclin-1	It plays a central role in autophagy and acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.
ATG12	Autophagy-related protein 12	Ubiquitin-like protein involved in autophagy vesicle formation.
ATG5	Autophagy protein 5	Involved in autophagic vesicle formation.
ATG7	Autophagy-related protrein 7	E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport and autophagy. It activates ATG12 for its conjugation with ATG5.
P62/SQSTM1	Sequestosome-1/Ubiquitin-binding protein p62	Autophagy receptor required for selective macroautophagy (aggrephagy). It functions as a bridge between polyubiquitinated cargo and autophagosomes. It interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family.
RAB7A	Ras-related protein Rab-7a	Key regulator in endo-lysosomal trafficking. It plays a role in the fusion of phagosomes with lysosomes.
GABARAP	Gamma-aminobutyric acid receptor-associated protein	Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage of autophagosome maturation.
Hepatocyte markers
AFP	Alpha-fetoprotein	It binds copper, nickel and fatty acids as well as serum albumin, and bilirubin less well than.
ALB	Serum albumin	The main protein of plasma having a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs.
TTR	Transthyretin	Thyroid hormone-binding protein.
Housekeeping genes
TUBB	Tubulin beta chain	Tubulin is the major constituent of microtubules.
HIST1H3A	Histone H3.1	Core component of nucleosome.