TABLE 2.
Primers and sequences in this study.
| Primers | Primer sequences (5′-3′) | Target |
| clpB-up-F | AACGACGGCCAGTGCCAAGCTCAATGAGTATGGCGTGGCGTA | Upstream fragment of clpB |
| clpB -up-R | CAATGACAGGATCGAAACGTACTCATCGCCTAACTC | Upstream fragment of clpB |
| clpB-down-F | GATGAGTACGTTTCGATCCTGTCATTGGCCGTGAC | Downstream fragment of clpB |
| clpB-down-R | CGGTACCCGGGGATCCTCTAGCGTTCCTTGGAAGCTGCATCG | Downstream fragment of clpB |
| dnaK-up-F | AACGACGGCCAGTGCCAAGCTGACGTGCAGTAGGAATTGACCTTGG | Upstream fragment of dnaK |
| dnaK-up-R | ATGCGCTGGAACTCTGCACGGGTCAACGATACGCTGATCCCAGTCGT | Upstream fragment of dnaK |
| dnaK-down-F | ATCAGCGTATCGTTGACCCGTGCAGAGTTCCAGCGCATCAC | Downstream fragment of dnaK |
| dnaK-down-R | CGGTACCCGGGGATCCTCTAGGACAGACCGGAGCCGTCCTGAATG | Downstream fragment of dnaK |
| clpB-F | AAACAGAATTAATTAAGCTTATGAGTTCATTCAATCCAACTACCA | ORF of clpB |
| clpB-R | GTACCCGGGGATCCTCTAGATTAATGATGATGATGATGATGATGGACCG CCTTGGAAACGTCGAGCTTC | ORF of clpB |
| dnaK-F | AACAGAATTAATTAAGCTTATGGGACGTGCAGTAGGAATT | ORF of dnaK |
| dnaK-R | TTAATGATGATGATGATGATGATGCTTCTTATCCTCACCATTGTC | ORF of dnaK |
| pXMJ19 check-F | CGGCTCGTATAATGTGTGGA | Recombinant pXMJ19 vector detecting |
| pXMJ19 check-R | ATCTTCTCTCATCCGCCAAA | Recombinant pXMJ19 vector detecting |
| M13-F | CGCCAGGGTTTTCCCAGTCACGAC | Recombinant pK18mobsacB vector detecting |
| M13-R | GAGGGGATAACAATTTCACACAGG | Recombinant pK18mobsacB vector detecting |