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Frontiers in Immunology logoLink to Frontiers in Immunology
. 2020 Jul 17;11:1521. doi: 10.3389/fimmu.2020.01521

Corrigendum: FB5P-seq: FACS-Based 5-Prime End Single-Cell RNA-seq for Integrative Analysis of Transcriptome and Antigen Receptor Repertoire in B and T Cells

Noudjoud Attaf 1,, Iñaki Cervera-Marzal 1,†,, Chuang Dong 1, Laurine Gil 1, Amédée Renand 2, Lionel Spinelli 1, Pierre Milpied 1,*
PMCID: PMC7380104  PMID: 32765526

In the original article, there was a mistake in Table S3 as published. The sequence of Read2_SP, the Read2 sequencing primer, was incorrect. The corrected Table S3 appears below.

Table S3.

Primers used in FB5P-seq.

Primer name Function in FB5P-seq Sequence 5-3
(dT)30_Smarter Prime Reverse Transcription at 3′ ends of polyadenylated mRNA and introduce PCR handle TGCGGTATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTVN
TSO_BCx_UMI5_TATA Perform template switching and introduce 8 nt. well-specific barcode & 5 nt. UMI AGACGTGTGCTCTTCCGATCTXXXXXXXXNNNNNTATArGrGrG
Satija_PCR Forward primer for LD-PCR amplification of cDNA libraries AGACGTGTGCTCTTCCGATCT
SmarterR Reverse primer for LD-PCR amplification of cDNA libraries TGCGGTATCTAAAGCGGTGAG
i7_BC1 Forward primer for 5′-end enrichment of tagmented library and incorporation of plate-specific barcode CAAGCAGAAGACGGCATACGAGATCCTGGTAGGTGACTGGAG
TTCAGACGTGTGCTCTTCCGATCT
i7_BC2 CAAGCAGAAGACGGCATACGAGATTAAGCATGGTGACTGGAG
TTCAGACGTGTGCTCTTCCGATCT
i7_BC3 CAAGCAGAAGACGGCATACGAGATAGATGTGCGTGACTGGAG
TTCAGACGTGTGCTCTTCCGATCT
i7_BC4 CAAGCAGAAGACGGCATACGAGATGTCGAGCAGTGACTGGAG
TTCAGACGTGTGCTCTTCCGATCT
i7_BC5 CAAGCAGAAGACGGCATACGAGATGAATTGCTGTGACTGGAG
TTCAGACGTGTGCTCTTCCGATCT
i7_BC6 CAAGCAGAAGACGGCATACGAGATAAGCAACTGTGACTGGAG
TTCAGACGTGTGCTCTTCCGATCT
i7_primer Forward primer for amplification of sequencing library CAAGCAGAAGACGGCATACGA
Read1_SP Read1 sequencing primer TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG
i7_SP Index Read i7 sequencing primer AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
Read2_SP Read2 sequencing primer GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.


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