In the original article, there was a mistake in Table S3 as published. The sequence of Read2_SP, the Read2 sequencing primer, was incorrect. The corrected Table S3 appears below.
Table S3.
Primer name | Function in FB5P-seq | Sequence 5′-3′ |
---|---|---|
(dT)30_Smarter | Prime Reverse Transcription at 3′ ends of polyadenylated mRNA and introduce PCR handle | TGCGGTATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTVN |
TSO_BCx_UMI5_TATA | Perform template switching and introduce 8 nt. well-specific barcode & 5 nt. UMI | AGACGTGTGCTCTTCCGATCTXXXXXXXXNNNNNTATArGrGrG |
Satija_PCR | Forward primer for LD-PCR amplification of cDNA libraries | AGACGTGTGCTCTTCCGATCT |
SmarterR | Reverse primer for LD-PCR amplification of cDNA libraries | TGCGGTATCTAAAGCGGTGAG |
i7_BC1 | Forward primer for 5′-end enrichment of tagmented library and incorporation of plate-specific barcode | CAAGCAGAAGACGGCATACGAGATCCTGGTAGGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT |
i7_BC2 | CAAGCAGAAGACGGCATACGAGATTAAGCATGGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT |
|
i7_BC3 | CAAGCAGAAGACGGCATACGAGATAGATGTGCGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT |
|
i7_BC4 | CAAGCAGAAGACGGCATACGAGATGTCGAGCAGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT |
|
i7_BC5 | CAAGCAGAAGACGGCATACGAGATGAATTGCTGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT |
|
i7_BC6 | CAAGCAGAAGACGGCATACGAGATAAGCAACTGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT |
|
i7_primer | Forward primer for amplification of sequencing library | CAAGCAGAAGACGGCATACGA |
Read1_SP | Read1 sequencing primer | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
i7_SP | Index Read i7 sequencing primer | AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC |
Read2_SP | Read2 sequencing primer | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.