Table S3.

Primers used in FB5P-seq.

Primer name	Function in FB5P-seq	Sequence 5′-3′
(dT)30_Smarter	Prime Reverse Transcription at 3′ ends of polyadenylated mRNA and introduce PCR handle	TGCGGTATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTVN
TSO_BCx_UMI5_TATA	Perform template switching and introduce 8 nt. well-specific barcode & 5 nt. UMI	AGACGTGTGCTCTTCCGATCTXXXXXXXXNNNNNTATArGrGrG
Satija_PCR	Forward primer for LD-PCR amplification of cDNA libraries	AGACGTGTGCTCTTCCGATCT
SmarterR	Reverse primer for LD-PCR amplification of cDNA libraries	TGCGGTATCTAAAGCGGTGAG
i7_BC1	Forward primer for 5′-end enrichment of tagmented library and incorporation of plate-specific barcode	CAAGCAGAAGACGGCATACGAGATCCTGGTAGGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT
i7_BC2		CAAGCAGAAGACGGCATACGAGATTAAGCATGGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT
i7_BC3		CAAGCAGAAGACGGCATACGAGATAGATGTGCGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT
i7_BC4		CAAGCAGAAGACGGCATACGAGATGTCGAGCAGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT
i7_BC5		CAAGCAGAAGACGGCATACGAGATGAATTGCTGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT
i7_BC6		CAAGCAGAAGACGGCATACGAGATAAGCAACTGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT
i7_primer	Forward primer for amplification of sequencing library	CAAGCAGAAGACGGCATACGA
Read1_SP	Read1 sequencing primer	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG
i7_SP	Index Read i7 sequencing primer	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
Read2_SP	Read2 sequencing primer	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT