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. Author manuscript; available in PMC: 2020 Jul 24.
Published in final edited form as: J Vis Exp. 2019 Oct 15;(152):10.3791/60275. doi: 10.3791/60275

Figure 2: Co-culture interaction assays uncover siderophore-mediated inhibition of CoNS by Corynebacterium propinquum.

Figure 2:

(A) Monoculture of CoNS (target organism) inoculated on a BHI bioassay plate. (B-D) Co-cultures between different strains of Corynebacterium spp. (test organisms, left) and the same strain of CoNS (target organism, right) inoculated on BHI bioassay plates. Each panel is a representative image showing interactions with (B) no inhibition (score = 0), (C) weak inhibition (score = 1), or (D) strong inhibition (score = 2). (E) Comparison of interactions between Corynebacterium pseudodiphtheriticum (siderophore non-producer) or Corynebacterium propinquum (siderophore producer) with the same strain of CoNS on BHI media (BHI) and BHI media supplemented with 200 μM FeCl3 (BHI + Iron).