Table 3.
Features of technique types for plant virus detection and diagnostics.
Color intensity is proportional to positive qualification.
ELISA, enzyme-linked immunosorbent assay, PCR, polymerase chain reaction; qPCR, quantitative PCR, LAMP, loop-mediated isothermal amplification, RPA, recombinase polymerase amplification; and HTS, high-throughput sequencing.
a
Versatility. Ability for different detection levels (family, genus, species, strain or isolate).
b
Multiplexing. Ability to perform parallel analysis (analyze several viruses simultaneously).
c
Ability to analyze many samples simultaneously.
d
On-site. Ability to detect viruses on field with portable devices.
e
Only nanopore sequencing is portable among the HTS techniques.