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. 2020 May 16;295(29):9838–9854. doi: 10.1074/jbc.RA120.012748

Figure 4.

Figure 4.

Time course pelleting assay of preformed amyloid fibrils incubated with full-length sHSP constructs. α-Syn fibrils (200 μm) were formed for 48 h at 37 °C, and apoC-II fibrils (56 μm) were formed for 72 h at 30 °C. Fibrils were then incubated with the indicated sHSP construct at 10 μm (for α-syn) or 12.8 μm (for apoC-II) for 0–6 h. Following this incubation, each sample was centrifuged as described above. The supernatant (Sup), sucrose (Suc), and pellet (P) layers were collected and analyzed by SDS-PAGE alongside the total (T) uncentrifuged samples. A–H, preformed α-syn fibrils incubated with αB-C WT (A), WT Hsp27 (C), Hsp27-3D (E), and Hsp27-3DΔCTR (G) and preformed apoC-II fibrils incubated with αB-C WT (B), WT Hsp27 (D), Hsp27-3D (F), and Hsp27-3DΔCTR (H). I and J, preformed α-syn or apoC-II fibrils and sHSP constructs incubated alone for 6 h at identical concentrations to those described above.