Figure 5.
Analysis of the interaction between IsdB NEAT1-NEAT2 and its derivatives, NEAT1 and NEAT2, with Vn by SPR. Twofold linear dilution series (2.5–160 nm) of Vn were injected over the IsdB NEAT1-NEAT2 or its derivatives, NEAT1 and NEAT2, immobilized on the surface of a CM5 sensor chip. The sensorgrams obtained were normalized versus the response obtained when Vn was flowed over uncoated chips. The affinity was calculated from curve fitting to a plot of the response unit values at the steady state (RUmax) against increasing concentrations of Vn (left). Shown is one representative of three experiments.