Figure 7.
Adhesion and internalization of S. aureus cells. Bacterial strains were grown overnight in BHI or RPMI medium, added to HeLa (A and B) or HUVEC (C and D) cell monolayers, and tested for adhesion (A and C) or internalization (B and D). The experiments were performed in DMEM for 2 h at 37 °C. The effects of αvβ3 mAb LM609 and cilengitide on bacterial adherence are also reported. The inocula and adherent and internalized bacteria were quantified by viable counting. Error bars show S.D. of the means from three independent determinations performed in triplicate with similar results (Student's t test; *, p < 0.05).