Table 2.
Plasmids
| Plasmid | Feature | Markera | Source or reference |
|---|---|---|---|
| pQE30 | E. coli vector for the expression of hexa-His-tagged recombinant proteins | Ampr | Qiagen |
| pQE30::isdB NEAT1-NEAT2 | pQE30 encoding residues 48–480 of IsdB NEAT1-NEAT2 protein | Ampr | 61 |
| pQE30::rIsdB-NEAT1 (144–270) | pQE30 derivative encoding the NEAT1 domain of IsdB NEAT1-NEAT2 protein from S. aureus SH1000 | Ampr | This study |
| pQE30::rIsdB-NEAT2 (334–458) | pQE30 derivative encoding the NEAT2 domain of IsdB protein from S. aureus SH1000 | Ampr | This study |
a Ampr, ampicillin resistance.