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Phenotypic analysis of thiL deletion in P. aeruginosa.A, a serial dilution of the thiL deletion mutant (ΔthiL) of P. aeruginosa was plated on LB agar and showed no growth after a 24-h incubation at 37 °C. B, TPP supplementation (1 mm) in LB agar rescued the growth defect of ΔthiL.