Figure 8.

The third step of CYP51 reaction proceeds via the compound I mechanism. A, catalytic parameters of WT and D231A/H314A human CYP51 with lanosterol and its 14α-carboxaldehyde intermediate as substrates. Points are shown as means of two determinations ± S.D. Examples of HPLC profiles are shown in Fig. S6. B, proton relay network in CYP51 catalysis: closed in ligand-free and inhibitor-bound structures (4UHI), open (active) upon substrate binding (6FMO), and destroyed by mutagenesis (6UEZ).