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. 2020 Jul 17;11:1488. doi: 10.3389/fimmu.2020.01488

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Copyright © 2020 Santana, Pitanga, de Santana, Zanette, Vieira, Yahouédéhou, Adanho, Viana, Luz, Borges and Goncalves.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DPPH scavenging activity of different concentrations of hydroxyurea after 60 min of incubation. Antioxidant activity was measured by scavenging of the DPPH free radical using the HU concentrations 3.13 μM (A), 6.25 μM (B), 12.5 μM (C), 25 μM (D), 50 μM (E), 100 μM (F), 200 μM (G), 400 μM (H) and 800 μM (I). Results correspond to the mean ± standard deviation of four independent experiments. BHT and L-ascorbate were used as reference antioxidant compounds. HU, hydroxyurea; L-Asc, L-ascorbate; BHT, butylated hydroxytoluene. Statistical significance determined by one-way ANOVA, p < 0.0001, followed by Tukey's post-hoc test: HU vs. BHT: *p < 0.05, **p < 0.01, ***p < 0.001; HU vs. L-Asc, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001.