Skip to main content
. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: ACS Chem Neurosci. 2020 Jun 18;11(13):2019–2030. doi: 10.1021/acschemneuro.0c00324

Figure 5.

Figure 5.

Concentration-dependent receptor binding assays for MP-IV-010. A) GABAAR; rat brain homogenate was incubated with 3H-flunitrazepam; B) κ-opioid receptor; cell lysate from stably transfected HEK cells was incubated with 3H-U69593; C) σ2 receptor; cell lysate from stably transfected HEK cells was incubated 3H-ditolylguanidine. (n = 6); D) Inhibition at 10 μM MP-IV-010: μ-opioid receptor; cell lysate from stably transfected HEK cells was incubated with 3H-DADLE; δ-opioid receptor; cell lysate from stably transfected HEK cells was incubated with 3H-DAMGO; hERG; cell lysate from stably transfected HEK cells was incubated with 3H-dofetilide. Data are presented as mean and SEM (n = 8). Non-linear regression was used to determine IC50 values.