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. Author manuscript; available in PMC: 2020 Jul 24.
Published in final edited form as: Nat Chem Biol. 2019 Apr 15;15(5):472–479. doi: 10.1038/s41589-019-0267-9

Figure 4. Structure guided re-selection.

Figure 4.

(a) Sequences of Mango-III, two structure-guided randomized pools (N, randomized positions), and four functionally reselected variants colored according to Mango-III structural elements (top). iMango-III is “Variant 58%”. (b) Reselection workflow. Equally mixed, randomized pools were first selected for high affinity binders through two rounds of SELEX. Resulting sequences underwent seven rounds of microfluidic droplet-based selection by individualizing each sequence in droplets (orange), thermocycling off chip, re-injected into a second chip to fuse with in vitro transcription mixture (yellow) followed by collection and incubation at 37˚C to amplify RNA, and transfer to a third chip for sorting based on fluorescence intensity (green).