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. Author manuscript; available in PMC: 2020 Jul 24.
Published in final edited form as: Nat Chem Biol. 2019 Apr 15;15(5):472–479. doi: 10.1038/s41589-019-0267-9

Figure 6. Photophysical properties of Mango-III and variants.

Figure 6.

(a) Normalized absorbance spectra of the TO1-Biotin complexes of Mango-III and iMango-III (black and green, respectively). (b) Normalized fluorescence emission spectra of the TO1-Biotin complexes of Mango-III and iMango-III (black and green, respectively). Mango-III(A10U) and iMango-III(U10A) exhibit excitation and emission spectra indistinguishable from those of Mango-III and iMango-III, respectively (Supplementary Figure 9). (c) Fluorescence intensity of four aptamer-TO1-Biotin complexes as a function of D2O percentage. (d) Fluorescence lifetime of four aptamer-TO1-Biotin complexes in 100% water or 96.6% D2O. Individual data points, mean of three replicates, and standard deviations are shown (Supplementary Table 7).