TABLE 1.
Culture | Survival rate (%)a
|
|||
---|---|---|---|---|
Wild-type strain |
Δpox Δlox
mutant |
ΔmntR
mutant |
ΔperR
mutant |
|
Static culture | 30 ± 3.27* | 0.02 ± 0.01* | ND | ND |
Anaerobic culture | ||||
Nonprepulse | 0.21 ± 0.07 | 0.23 ± 0.06 | 7.94 ± 3.18 | 75 ± 11 |
Prepulse with 40 μM H2O2 | 77 ± 33 | 66 ± 12 | 62 ± 26 | 86 ± 14 |
Prepulse with 100 μM H2O2 | 46 ± 4 | ND | ND | ND |
Strains were grown anaerobically in a 6-ml BHI culture, and then the survival percentages in 10 mM H2O2 were determined for all strains except for strains labeled with an asterisk, which were statically grown in a 40-ml culture and challenged with 20 mM H2O2. The survival percentage was calculated by dividing the number of CFU in the H2O2-treated culture by that in the untreated culture. The experiments were repeated three times with triplicate batch cultures each time. The results are averages ± SD from three independent experiments. ND, not determined.