Table 1. Effect of Ca2+ entry on AP waveform.
Table 1—source data 1. Effect of Ca2+ entry on AP waveform.
elife-54566-table1-data1.xlsx (16.5KB, xlsx)
| RMP (mV) |
AP threshold (mV) |
AP amplitude (mV) |
AP half-width (μs) |
AHP (mV) |
ADP (mV) |
|
|---|---|---|---|---|---|---|
| Control | –77.4 ± 2.5 | –67.1 ± 3.0 | 110.9 ± 12.5 | 612.7 ± 24.7 | 5.6 ± 2.1 | 7.6 ± 2.5 |
| TTA-P2 + isradipine | –76.0 ± 2.9 | –65.0 ± 4.1 | 109.3 ± 3.3 | 634.5 ± 49.6 | 3.2 ± 2.9 | 4.6 ± 3.3 |
| Paired t-tests (n = 5) | p=0.32 | p=0.30 | p=0.33 | p=0.61 | p=0.055 | p=0.039* |
| Control | –70.4 ± 1.6 | –59.3 ± 3.9 | 102.4 ± 1.8 | 753.8 ± 45.9 | 3.7 ± 3.8 | 6.5 ± 3.4 |
| EGTA | –71.8 ± 3.6 | –65.4 ± 1.8 | 103.5 ± 2.4 | 1031.5 ± 39.6 | 25.5 ± 4.3 | 11.7 ± 1.5 |
| Paired t-tests (n = 4) | p=0.69 | p=0.19 | p=0.27 | p=0.035* | p=0.043* | p=0.17 |
Overview of mean and s.e.m. of AP properties compared between control and toxin experiments recorded at the soma. RMP, resting membrane potential, AHP, fast afterhyperpolarization, ADP, afterdepolarization. The APs were elicited by large and brief current injections (~6 nA for 0.5 ms) to obtain temporally aligned APs between trials and image OGB-1 fluorescence (Figure 3). AP amplitude, AHP and ADP were measured relative to the AP threshold. If the AHP or ADP was not detectable as a local peak, the membrane potential at the time point as in control was used (see EGTA example Figure 9d). P values are results of two-tailed paired t-tests before and after toxin application. Data available in Table 1—source data 1.