Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Rac1 clone 23A8 (Mouse monoclonal) | Millipore | cat.05–389 | (1:500) |
| Antibody | Anti-RhoA (26C4) (Mouse monoclonal) | Santa-Cruz Biotechnology | cat.sc-418 | (1:200) |
| Antibody | anti-GAPDH (D16H11) XP (Rabbit monoclonal) | CST | cat.5174 | (1:3000) |
| Antibody | anti-DIA1 (Rabbit polyclonal) | Thermo | cat.PA5-21409 | WB (1:1500) IF (1:200) |
| Antibody | anti-ROCK1 (Rabbit polyclonal) | Thermo | cat.PA5-22262 | (1:100) |
| Antibody | anti-Rac-GTP (Mouse monoclonal) | New East Bio | cat.26903 | (1:100) |
| Antibody | Anti-mouse F(ab')2 Fragment Alexa Fluor 647 (Goat polyclonal) | Thermo | cat. A-21237 | (1:400) |
| Antibody | Anti-rabbit F(ab')2 Fragment Alexa Fluor 594 (Goat polyclonal) | Thermo | cat. A-11072 | (1:400) |
| Antibody | Anti-rabbit Alexa Fluor-488 (Donkey polyclonal) | Thermo | cat. A-21206 | (1:250) |
| Antibody | Anti-rabbit Alexa Fluor-594 (Goat polyclonal) | Thermo | cat. A-11012 | (1:250) |
| Antibody | Anti-rabbit IgG, HRP-linked (Goat polyclonal) | CST | cat.7074 | (1:10000) |
| Antibody | Anti-mouse IgG, HRP-linked (Horse polyclonal) | CST | cat.7076 | (1:10,000) |
| Strain, strain background (Lentivirus) | IncuCyte NucLight Red Lentivirus Reagent | Essen | Cat. 4625 | |
| Chemical compound, drug | Y-27632 | Sigma Aldrich | Cat.Y0503 | |
| Chemical compound, drug | GSK 269962 | Selleckchem | Cat.S7687 | |
| Chemical compound, drug | 4,6-Diamidino-2-phenylindole dihydrochloride (DAPI), stain | Sigma Aldrich | Cat.10236276001 | (1 µg/ml) |
| Chemical compound, drug | Rhodamine Phalloidin | Thermo | Cat. R415 | |
| Chemical compound, drug | Phalloidin- Alexa Fluor-488 | Thermo | Cat. A12379 | |
| Chemical compound, drug | Puromycin | Sigma Aldrich | Cat. P8833 | |
| Chemical compound, drug | Polibrene | Millipore | Cat.TR-1003-G | |
| Chemical compound, drug | Lipofectamine RNAiMax | Thermo | Cat.13778 | |
| Other | GST-Beads | Sigma Aldrich | G.4510 | |
| Other | Dulbecco’s Modified Eagle Medium (DMEM) | Sigma Aldrich | Cat.D6429 | |
| Other | FluoroBrite DMEM Media | Thermo | Cat. A1896701 | |
| Other | Fetal Bovine Serum (FBS) | Gibco | Cat.10270 | |
| Other | Collagen (rat tail) | Sigma Aldrich | Cat.11179179001 | |
| Cell line (Human) | MDA-MB-231 | ATCC | Cat.HTB-26 | Authenticated by the Beatson Institute, Glasgow, UK |
| Transfected Construct (Human) |
DIAPH1 siRNA SMART Pool | Dharmacon | cat. L-010347-00-0010 | |
| Recombinant DNA reagent | GST-rhotekin-RBD | Dr. Mike Olson gift (Beatson Institute, Glasgow, UK) | ||
| Recombinant DNA reagent | GST-PAK-CRIB | Dr. Piero Crespo gift (IBBTEC,University of Cantanbria, Spain) | ||
| Recombinant DNA reagent | mTFP-YFP RhoA activity probe | Prof. Olivier Pertz Gift (Institute of Cell Biology, Bern, Switzerland) | ||
| Recombinant DNA reagent | psPAX-2 | Prof. Olivier Pertz Gift | ||
| Recombinant DNA reagent | VsVg | Prof. Olivier Pertz Gift | ||
| Software, algorithm | Fiji | Schindelin et al., 2012 | https://imagej.net/Fiji | |
| Software, algorithm | OpenFOAM | Weller et al., 1998 | https://www.openfoam.com/ | |
| Software, algorithm | ParaView | Ayachit, 2015 | https://www.paraview.org/ | |
| Software, algorithm | Salome | Ribes and Caremoli, 2007 | https://www.salome-platform.org/ | |
| Software, algorithm | Python | https://www.python.org/ | ||
| Software, algorithm | SciPy | Virtanen et al., 2020 | https://www.scipy.org/ | |
| Software, algorithm | MatplotLib | Hunter, 2007 | https://matplotlib.org/ | |
| Software, algorithm | OpenCV | Bradski, 2000 | https://opencv.org/ | |
| Software, algorithm | DYVIPAC | Nguyen et al., 2015 | https://bitbucket.org/andreadega/dyvipac-python/src/master/ | |
| Software, algorithm | BioNetGen | Blinov et al., 2004; Harris et al., 2016 | https://www.csb.pitt.edu/Faculty/Faeder/?page_id=409 |