Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | C57BL/6n | Beijing Vital River Laboratory | Stock No.: 213 | |
| Genetic reagent (M. musculus) | ICR | Beijing Vital River Laboratory | Stock No.: 201 | |
| Genetic reagent (M. musculus) | BDF1 | This paper | / | The BDF1 hybrid mice were obtained from mating female C57BL/6n mice with male DBA/2 mice. |
| Genetic reagent (M. musculus) | DBA/2 | Beijing Vital River Laboratory | Stock No.: 214 | |
| Genetic reagent (M. musculus) | PWK/PhJ | Jackson Laboratory | Stock No.: 003715 | |
| Gene (Homo sapiens) | AAVS1 | GenBank | GeneID: 54776 | |
| Gene (M. musculus) | Actb | GenBank | GeneID: 11461 | |
| Gene (M. musculus) | Sox2 | GenBank | GeneID: 20674 | |
| Gene (M. musculus) | Rosa26 | GenBank | GeneID: 14910 | |
| Gene (M. musculus) | Cdx2 | GenBank | GeneID: 12591 | |
| Strain, strain background (Escherichia coli) | Trans109 | TransGen Biotech | CD301 | Competent cells |
| Cell line (Homo sapiens) | HCA2-hTERT | Gorbunova et al., 2002 (doi: 10.1074/jbc.M202671200) | Human fibroblast | |
| Cell line(Homo sapiens) | HEK 293FT | ATCC | RRID:CVCL_6911 | |
| Cell line (M. musculus) | E14 | ATCC | RRID:CVCL_C320 | Mouse ES cell line |
| Cell line (M. musculus) | Oct4-ΔPE-GFP transgenic C57BL/6 × PWK ESC | This paper | Mouse ES cell line | |
| Antibody | Anti-RPA2 (Rabbit polyclonal) | Abclonal | A2189 RRID:AB_2764207 |
WB (1:1000) |
| Antibody | Anti-NBS1 (Rabbit) |
Cell Signaling Technology | 3002 RRID:AB_331499 |
WB (1:1000) |
| Antibody | Anti-RAD50 (Rabbit polyclonal) | Abclonal | A3078 RRID:AB_2764881 |
WB (1:1000) |
| Antibody | Anti-RAD51 (Mouse polyclonal) | Abcam | ab88572 RRID:AB_2042762 |
IF (1:500), WB (1:1000) |
| Antibody | Anti-MRE11 (Rabbit polyclonal) | Abclonal | A2559 RRID:AB_2764447 |
WB (1:1000) |
| Antibody | Anti-53BP1 (Rabbit polyclonal) | Cell Signaling Technology | 4937S RRID:AB_10694558 |
IF (1:100), WB (1:1000) |
| Antibody | Anti-CtIP (Rabbit polyclonal) | Abcam | ab70163 RRID:AB_1209429 |
WB (1:1000) |
| Antibody | Anti-BRCA1 (Rabbit polyclonal) | Abclonal | A0212 RRID:AB_2757026 |
WB (1:1000) |
| Antibody | Anti-EXO1 (Rabbit polyclonal) | Abclonal | A6810 RRID:AB_2767391 |
WB (1:1000) |
| Antibody | Anti-XRCC2 (Rabbit polyclonal) | Abclonal | A1800 RRID:AB_2763839 |
WB (1:1000) |
| Antibody | Anti- TUBULIN (Rabbit polyclonal) | Abmart |
M20005 |
WB (1:2000) |
| Antibody | Anti-gamma H2AX (Rabbit polyclonal) | Cell Signaling Technology | 9718S RRID:AB_2118009 |
IF (1:500) |
| Recombinant DNA reagent | sgRNA-Cas9 plasmid | PMID:28524166 | Kind gift from Hui Yang lab | |
| Commercial assay or kit | Click-iT EdU Assay Kit | Invitrogen | C10634 | |
| Commercial assay or kit |
Comet assay | Trevigen | Cat. # 4250–050 K | |
| Chemical compound, drug | Farrerol | Sigma Aldrich | SML1389 | 0.05, 0.1, 1, 5, 10 μM |
| Chemical compound, drug | RS-1 | ApexBio | C3357 | 1, 7.5, 10 μM |
| Chemical compound, drug | SCR7 | Selleck | S7742 | 0.1, 1, 20 μM |
| Software, algorithm | ImageJ software | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
| Software, algorithm | GraphPad Prism software | GraphPad Prism (http://graphpad.com) | RRID:SCR_015807 | Version 6.0 |
| Software, algorithm | CASPLab software | CASPLab http://casp.sourceforge.net | RRID:SCR_007249 | |
| Other | DAPI stain | Abcam | ab104939 |