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. 2020 Jul 23;8(2):e000516. doi: 10.1136/jitc-2019-000516

Figure 3.

Figure 3

Prexasertib-induced changes in adaptive immunity. Paired pre-treatment and post-treatment PBMCs assessed by flow cytometry compared in 23 patients using a Wilcoxon matched-pairs signed-rank test. Lines in A and C-D show median and 95% CI. (A) CIBERSORT analysis to detect immune cell tumor infiltration among nine paired pre-treatment and post-treatment biopsies show an increase in naïve B-cells and resting memory CD4+T cells, and a decrease in the proportion of infiltrating resting mass was observed. (B) Among 12 available post-treatment core biopsies, high (above median) T-reg tumor infiltration was correlated with a significantly worse PFS (9.25 vs 3.5 months, p=0.007) using the Mantel-Cox log-rank test. (C) Decreased proportion of CD4+ and CD8+T cells per total viable cells observed from pre-treatment to post-treatment. (D) An increase in the proportion of regulatory T-cells and effector regulatory T-cells among total CD4+T-cells was observed post-treatment. eTreg, effector regulatory T-cell; PBMCs, peripheral blood mononuclear cells; Post-tx, post-treatment; PFS, progression-free survival; Treg, regulatory T-cell.