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. Author manuscript; available in PMC: 2021 Jul 21.
Published in final edited form as: Circulation. 2020 Apr 30;142(3):259–274. doi: 10.1161/CIRCULATIONAHA.119.044452

Figure 4: Enhanced α-dystroglycan glycosylation correlates with myocardial recovery.

Figure 4:

A) GC-MS analysis depicts similar levels of ribitol in donor and Post-R, with an increase in non-failing heart and Post-NR (n= donors-14, NR-20, R-7). B) Representative western blot from 7 independent experiments revealing enhanced levels of ribitol-5-phosphate cytidylyltransferase (ISPD) and similar fukutin-related protein (FKRP) protein levels in Post-R as compared to Post-NR. HSP60 is the lane loading control. C) and D) Densitometric analysis normalized to HSP60 of the western blots for ISPD and FKRP respectively. E) Representative western blot and F) corresponding densitometric analysis, showing enhanced glycosylation of α-dystroglycan (DAG1) in Post-R. n=7 for each group; p-value < 0.05 was considered significant; #p< 0.05, ##p<0.01, ###p<0.001 and ####p<0.0001 as compared to donors; *p< 0.05 compared to Post-NR.