Table 1.
Chemical reagents and drugs for primary neuronal culture
| Chemical | Concentration (mm) | Manufacturer, catalog # |
| NaCl | 116 | Millipore Sigma, S7653 |
| KCl | 5.4 | Millipore Sigma, P9541 |
| NaHCO3 | 26 | Millipore Sigma, S5761 |
| D-(+)-Glucose | 25 | Millipore Sigma, G8270 |
| NaH2PO4 | 2 | Millipore Sigma, 71505 |
| MgSO4 | 1 | Millipore Sigma, M7506 |
| Cysteine | 1.3 | Millipore Sigma, C6645 |
| EDTA | 0.5 | Millipore Sigma, E9884 |
| Kynurenate | 0.5 | Millipore Sigma, K3375 |
| Papain | 20 units/ml | Worthington, LS003126 |
| Penicillin/streptomycin | 7.70% | Millipore Sigma, P4333 |
| L-glutamine | 0.90% | Millipore Sigma, 59202C |
| Poly-L-lysine | 100 μg/ml | Millipore Sigma, P1399 |
| Laminin | 5 μg/ml | Millipore Sigma, L2020 |
| Insulin | 0.025 g/ml | Millipore Sigma, I2643 |
| Apotransferrin | 50 ml/ml | Millipore Sigma, T0178 |
| GDNF | 1 ng/ml | Millipore Sigma, SRP3200 |