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. 2020 Jul 20;15:1201–1212. doi: 10.2147/CIA.S254885

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© 2020 Liu et al.

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Estrogen negatively regulates Cxcl9 secretion in osteoblasts. (A) qPCR analysis of Cxcl9 mRNA in primary osteoblasts treated with vehicle, estradiol or fulvestrant (estrogen receptor antagonist). (B) Western blot of Cxcl9 in primary osteoblasts. (C) Cxcl9 concentrations assessed by ELISA in supernatant of primary osteoblasts treated with vehicle, estradiol or fulvestrant. n=5 per group. Data are shown as mean ± s.d. *P < 0.05, **P < 0.01 (Student’s t-test).