Figure 1.

STING has a strong impact on shaping the immunodominance of MVA-specific primary immune responses particularly acting on the immunodominant B8 antigen. (A) STING KO mice (STING KO) and WT-littermates (WT) were vaccinated on day 0 i. p. with 10⁷ IU MVA-P7.5-OVA. Seven days after priming antigen-specific CD8⁺ T cells were analyzed ex vivo by flow cytometry for their functionality by expression of (B) IFN-γ, (C) CD107a, and (D) MIP-1α. Anti-CD3 antibody (α-CD3) and β-galactosidase (β-Gal) peptides were used for stimulation as positive and negative controls, respectively. Data are represented as mean ± SD of n = 8 mice per group pooled from three independent experiments. Statistical significance (P); **P ≤ 0.01.