Figure 3.

STING is not relevant for developing long term memory and efficient secondary expansion of CD8⁺ T cell responses in prime/boost vaccination. (A) STING KO mice (STING KO) and WT-littermates (WT) were vaccinated i. p. with 10⁷ IU MVA-P7.5-OVA on day 0 (prime) and 28 (boost). Five days after the boost antigen-specific CD8⁺ T cells were analyzed ex vivo by flow cytometry for their functionality by expression of (B) IFN-γ, (C) CD107a, and (D) MIP-1α. Anti-CD3 antibody (α-CD3) and β-galactosidase (β-Gal) peptides were used for stimulation as positive and negative controls, respectively. Data are represented as mean ± SD of n = 9 mice per group pooled from three independent experiments.