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. 2020 Jul 16;11:1458. doi: 10.3389/fimmu.2020.01458

Figure 4.

Figure 4

Re-activation of antigen-specific CD8+ T cells by infected or cross-presenting STING KO BMDC is not impaired. (A) BMDCs from STING KO mice (STING KO) or wildtype-littermates (WT) were infected with MVA-PK1L-OVA at MOI 1 (inf) or mock infected (mock) for 12 h. (B) MHC I-mismatched wildtype feeder cells (feeder WT) expressing STING were infected with MVA-PK1L-OVA at MOI 1 (inf) or mock infected (mock) for 12 h, treated with PUVA, washed and co-cultured with BMDCs derived from either STING KO mice (KO) or WT littermates (WT). (C) MHC I-mismatched STING KO (feeder KO) were infected with MVA-PK1L-OVA at MOI 1 (inf) or mock infected (mock), treated with PUVA, washed and co-cultured with BMDCs generated from either STING KO (KO) or wildtype mice expressing STING (WT). (A) BMDCs were analyzed 12 h post-infection or (B,C) 12 h post co-cultivation for their ability to activate CD8+ T cell lines to produce IFN-γ as determined by ICS followed by FACS analysis. CD8+ T cell activation was determined as frequency of IFN-γ expressing T cells specific for the indicated peptides derived from OVA or MVA antigens (B8, A3, K3, A19, D13). Data are represented as mean ± SD of (A) n = 3 or (B,C) n = 2 BMDC preparations from individual mice per group pooled from (A) three or (B,C) two independent experiments.