Figure 5.

STING in BMDC is crucial for efficient antigen processing and presentation while STING in feeder cells increases the size of the DC pool available for cross-presentation. (A,B) GM-CSF-BMDCs generated from STING KO mice (KO) or WT-littermates (WT) were infected with MVA-PK1L-OVA at MOI 1 (inf) or mock infected (mock). At 12 h post-infection SIINFEKL/K^b surface expression was determined. (C,D) The SIINFEKL/K^b loading ability of cross-presenting BMDCs was analyzed after co-culturing MVA-infected STING KO (feeder KO) or WT Cloudman (CM) feeder cells (feeder WT) with either STING KO (presenter KO) or WT BMDCs (presenter WT) for 20 h. Data are representative for one of two independent experiments and are presented as (A,C) frequencies (%) or (B,D) mean fluorescent intensities (MFI) and represent the mean ± SD of n = 3 BMDC preparations from individual mice per group. Statistical significance (P); *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.