Figure 6.

STING KO and WT BMDCs showed comparable phagocytosis activity and endosomal function. (A) GM-CSF-BMDCs were generated from STING KO (KO) mice or wildtype littermates (WT). The phagocytic activity was analyzed using (A,C) OVA-AF488 or (B,D) DQ-OVA at 4 and 37°C. In addition, DQ-OVA (B,D) allowed to measure proteolytic activity resulting in increased fluorescence. Data are presented as (A,B) frequencies (%) or (C,D) mean fluorescent intensities (MFI) and represent the mean ± SD of n = 3 BMDC preparations from individual mice per group pooled from three independent experiments.