Figure 8.

STING is crucial for the induction of type I interferon in vitro. (A) GM-CSF-BMDCs generated from STING KO mice or WT-littermates were either directly infected with MVA-PK1L-OVA at MOI 1 or mock infected or (B) left uninfected, but co-cultivated with feeder cells which were infected with MVA-PK1L-OVA at MOI 1 for 12 h or left uninfected (mock). Supernatants were collected at (A) 12 h post-infection or (B) 12 h post co-cultivation and (A,B) concentrations of IFN-α and -β were determined by ELISA. In the co-culture setting, supernatants from co-cultures with either infected STING KO (feeder KO) or wildtype feeder cells (feeder WT) with either uninfected STING KO (presenter KO) or WT BMDCs (presenter WT) were taken at 12 h post co-culturing and tested. Data represent the mean ± SD of (A) n = 4 and (B) n = 3 BMDC preparations from individual mice per group pooled from three independent experiments. Statistical significance (P); **P ≤ 0.01; ***P ≤ 0.001; n. d. = not detected.