Figure 9.

MVA-vaccinated STING KO mice show a decreased cytokine and chemokine response. C57BL/6 mice (WT) and STING KO (KO) were i. p. infected with 1 × 10⁸ IU MVA-PH5-OVA. After 6 h of infection, spleens were harvested, cell suspensions prepared and (A) cytokines or (B) chemokines were determined in spleen supernatants by cytoplex assay. Data from infected groups (WT inf, KO inf) are represented as mean ± SD of n = 4-6 (cytokines) and n = 3 (chemokines) mice per group pooled from two independent experiments. Naïve STING KO (KO naïve) and wildtype (WT naïve) mice were used as control and data are depicted as mean ± SD of n = 2-3 (cytokines) and n = 2 (chemokines) mice per group. Statistical significance (P); *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.