Figure 1.
Comparison of antibody and nuclear staining between different fixation procedures for IMC within a single tissue block. (A) Comparison of the staining intensity of each antibody depending on the fixation conditions, based on the maximum signal threshold in MCDTM viewer. Black bars indicate median ± IQR. Each gray dot represents an individual ROI. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by Kruskal-Wallis test with Dunn's test for multiple comparisons. (B) The markers CD45 and CD3 are representative for the variations observed with the tested incubation conditions. The minimum signal threshold of 2 dual count was set for the nuclear staining, while that was 1.5 for the immune markers.