Table 1.

The 34-marker panel for imaging mass cytometry on snap-frozen tissue.

	Antigen	Tag	Clone	Supplier	Cat.	Dilution
1	CD45	89Y	HI30	Flui	3089003B	1/50
2	D2-40	115In	D2-40	BioL	916606	1/50
3	FOXp3	142Nd	D608R	CST	12653BF	1/50
4	CD69	144Nd	FN50	Flui	3144018B	1/50
5	CD4	145Nd	RPA-T4	Flui	3145001B	1/50
6	CD8a	146Nd	RPA-T8	Flui	3146001B	1/50
7	Collagen I	147Sm	Polyclonal	Millipore	AB758	1/100
8	α-SMA	148Nd	1A4	CST	CST5685BF	1/200
9	CD31	149Sm	8 9C2	CST	CST3528BF	1/100
10	E-Cadherin	150Nd	24 E 10	CST	CST3195BF	1/50
11	CD123	151Eu	6H6	Flui	3151001B	1/50
12	CD7	153Eu	CD7-6B7	Flui	3153014B	1/100
13	CD163	154Sm	GHI/61	Flui	3154007B	1/100
14	CD103	155Gd	EPR4166	Abcam	ab221210	1/50
15	CD127	156Gd	R34.34	Beckman	18LIQ494	1/50
16	CD122	158Gd	TU27	BioL	339015	1/25
17	CD68	159Tb	KP1	Flui	3159035D	1/200
18	CD5	160Gd	UCHT2	BioL	300627	1/25
19	CD20	161Dy	H1	Flui	3161029D	1/50
20	CD11c	162Dy	Bu15	Flui	3162005B	1/50
21	CD45	163Dy	D9M81	CST	13917BF	1/200
22	CD161	164Dy	HP-3G10	Flui	3164009B	1/50
23	CD117	165Ho	104D2	BioL	313202	1/50
24	Ki-67	166Er	D3B5	CST	CST 9129BF	1/200
25	CD27	167Er	O323	Flui	3167002B	1/50
26	HLA-DR	168Er	L243	BIoL	307651	1/800
27	CD45RA	169Tm	HI100	Flui	3169008B	1/100
28	CD3	170Er	UCHT1	Flui	3170001B	1/100
29	CD28	171Yb	CD28.2	BioL	302937	1/50
30	CD38	172Yb	HIT2	Flui	3172007B	1/100
31	CD45RO	173Yb	UCHL1	BioL	304239	1/50
32	CD57	174Yb	HNK-1/Leu-7	Abcam	Ab212403	1/100
33	Vimentin	175Lu	D21H3	CST	CST5741BF	1/200
34	CD56	176Yb	NCAM16.2	Flui	3176008B	1/50

Flui, Fluidigm; CST, cell signaling technology; Biol, Biolegend.