Table 2.

The experimental set up of the testing of the various conditions.

Slide Nr	Slide drying			Fixation					Panel incubation
	3 min	30 min	1 h	Methanol	1% PFA	1% PFA + methanol	Acetone	4% PFA	5 h at RT	Overnight at 4°C
1	+	–	–	–	–	+	–	–	–	+
2	–	+	–	+	–	–	–	–	–	+
3	–	+	–	–	+	–	–	–	–	+
4	–	+	–	–	–	+	–	–	–	+
5	–	+	–	–	–	–	+	–	–	+
6	–	+	–	–	–	–	–	+	–	+
7	–	+	–	–	–	+	–	–	+	–
8*	–	–	+	–	–	+	–	–	–	+

^*Conditions applied to slide #8 represent the optimal staining protocol.