Table 3.
The estimated signal-to- background ratio of the antibodies in the optimal staining protocol.
| Antigen | Channel | Adjusted threshold min | Default threshold max | Estimated signal-to-background ratio* |
|---|---|---|---|---|
| CD3 | 170Er | 1.00 | 17.94 | 17.94 |
| CD4 | 145Nd | 1.50 | 5.41 | 3.61 |
| CD5 | 160Gd | 1.50 | 4.17 | 2.78 |
| CD7 | 153Eu | 1.50 | 21.97 | 14.65 |
| CD8a | 146Nd | 1.50 | 4.60 | 3.07 |
| CD11c | 162Dy | 1.00 | 4.42 | 4.42 |
| CD20 | 161Dy | 2.00 | 5.08 | 2.54 |
| CD27 | 167Er | 1.00 | 4.93 | 4.93 |
| CD28 | 171Yb | 1.00 | 3.99 | 3.99 |
| CD31 | 149Sm | 2.00 | 18.62 | 9.31 |
| CD38 | 172Yb | 1.50 | 12.01 | 8.01 |
| CD45_1 | 89Y | 1.50 | 9.66 | 6.44 |
| CD45RA | 169Tm | 2.00 | 32.74 | 16.37 |
| CD45RO | 173Yb | 3.00 | 17.60 | 5.87 |
| CD56 | 176Yb | 5.00 | 61.80 | 12.36 |
| CD57 | 174Yb | 5.00 | 22.25 | 4.45 |
| CD68 | 159Tb | 5.00 | 66.47 | 13.29 |
| CD69 | 144Nd | 1.50 | 4.20 | 2.80 |
| CD103 | 155Gd | 1.50 | 4.16 | 2.77 |
| CD117 | 165Ho | 1.00 | 4.58 | 4.58 |
| CD123 | 151Eu | 1.50 | 6.46 | 4.31 |
| CD127 | 156Gd | 2.00 | 4.32 | 2.16 |
| CD161 | 164Dy | 1.50 | 7.42 | 4.95 |
| CD163 | 154Sm | 2.00 | 23.10 | 11.55 |
| Collagen I | 147Sm | 3.00 | 46.60 | 15.53 |
| D2-40 | 115In | 3.00 | 6.56 | 2.19 |
| Vimentin | 175Lu | 5.00 | 94.23 | 18.85 |
| E-Cadherin | 150Nd | 1.50 | 7.89 | 5.26 |
| HLA-DR | 168Er | 3.00 | 41.36 | 13.79 |
| Ki-67 | 166Er | 2.00 | 11.69 | 5.85 |
| α-SMA | 148Nd | 3.00 | 46.20 | 15.40 |
| CD122 | 158Gd | 1.00 | 3.48 | 3.48 |
| DNA1 | 191Ir | 3.00 | 44.85 | 14.95 |
| FOXp3 | 142Nd | 2.00 | 3.10 | 1.55 |
| CD45_2 | 163Dy | 2.00 | 14.10 | 7.05 |
| DNA2 | 193Ir | 3.00 | 76.37 | 25.46 |
*
Estimated Signal-to-background was defined as Default Threshold Max/Adjusted Threshold Min.