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. 2020 Jul 15;8:491. doi: 10.3389/fcell.2020.00491

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Copyright © 2020 Chen, An, Huang, Xuan, Zhang, Ye, Luo, Xuan, He, Zheng and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Betaine protected the mitochondria in NIH 3T3 cells against oxidative damage. (A) Fluorescence staining of JC-1 for mitochondria membrane potential. Red: mitochondrial aggregates. Green: mitochondrial monomers. Scale bar = 100 μm. (B) The ratio of red to green fluorescence, n = 3. CCCP: positive control. BET: betaine. (C) Schematic showing the mitochondrial protective effect of BET against H₂O₂ damage. Statistical differences were performed using ANOVA, ***p < 0.001.