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. 2020 Jul 14;11:1479. doi: 10.3389/fmicb.2020.01479

FIGURE 2.

FIGURE 2

Advanced glycation end products (AGEs) enhance biofilm formation of S. aureus strains. (A) Biofilm-forming capability of S. aureus with varying AGE doses. Photograph of S. aureus Newman strain grown 24 h at different AGEs concentration after crystal violet staining, and the absorbance was measured at 570 nm (OD570). Three biological replicates for each assay. Photograph of S. aureus strain (B) Newman, (C) SA03, and (D) NCTC8325 grown 24 h at 50 μg/ml bovine serum albumin (BSA) group (control group), 40 mmol/l glucose, and 50 μg/ml AGEs-BSA group after crystal violet staining. The absorbance of different S. aureus strains was measured at 570 nm (OD570). #compared with 0 μg/ml, P < 0.05; △ compared with trypsin soybean broth (TSB) group, P < 0.05; ∗compared with TSB-BSA group, P < 0.05.