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. 2020 Jul 14;11:1479. doi: 10.3389/fmicb.2020.01479

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Copyright © 2020 Xie, Liu, Li, Luo, Yuan, Chen, Liang, Shen, Li, Huang and Duan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biofilm structures and biomass of S. aureus strain Newman cultured in trypsin soybean broth (TSB) with 50 μg/ml advanced glycation end product (AGE)-bovine serum albumin (BSA) and other control, measured by confocal laser scanning microscopy (CLSM). (A) Live/Dead staining images. The viable bacteria were stained by SYTO9 (green) and PI stained the dead bacteria (red). The images were showed after merging (green, red, and yellow). (B) Comparison of biofilm thickness (*P < 0.05 compared with TSB group; #P < 0.05 compared with TSB + BSA group).