FIGURE 6.

Advanced glycation end products (AGEs) induced e-DNA release by positively affected sigB transcription and downregulated lrg A expression. (A) Photograph of Newman strain and Newman-ΔsigB strain grown 24 h at TSB, TSB with 50 μg/ml BSA, and 50 μg/ml AGEs-BSA, after crystal violet staining. (B) The OD570 was detected (n = 3). *P < 0.05. (C) eDNA was extracted and determined. AGEs-BSA obviously increased the eDNA of the wild-type Newman, but had no effect on the eDNA of Newman-ΔsigB strain. *P < 0.05. (D) Reverse transcription quantitative PCR (RT-qPCR) detection of the expression level of lrgA gene in Newman and Newman-ΔsigB with or without AGE treatment. The lrgA transcription increased in Newman ΔsigB strain, and AGEs had no effect on the lrg A expression as that in Newman wild-type strain. *P < 0.05; **P < 0.01.