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. 2020 Jul 14;10:1126. doi: 10.3389/fonc.2020.01126

Figure 2.

Figure 2

METTL3 suppressed metastatic ability in TNBC cells. (A) qRT-PCR was used to detect METTL3 expression in MDA-MB-231 and MDA-MB-468 cells transfected with si-NC or si-METTL3. 18S was used as an internal control. (B) Western blot was used to detect METTL3 expression in MDA-MB-231 and MDA-MB-468 cells transfected with si-NC or si-METTL3. α-tubulin was used as a loading control. (C,D) Transwell assay was used to detect the migration and invasion ability in MDA-MB-231 and MDA-MB-468 cells with METTL3 transient knockdown (left panels). Relative fold change was shown as the proportion of the number of control cells transfected with si-NC (right panels). Original magnification, 100×. (E) Adhesion assay was used to detect the adhesion ability of MDA-MB-231 and MDA-MB-468 cells with METTL3 transient knockdown (left panels). Relative fold change was shown as the proportion of the number of control cells transfected with si-NC (right panels). Original magnification, 100×. *P < 0.05, **P < 0.01. Error bars represent the mean ± SD of three independent experiments.