Skip to main content
. 2020 Jul 14;10:1126. doi: 10.3389/fonc.2020.01126

Figure 3.

Figure 3

METTL3 overexpression-suppressed migration, invasion, and adhesion were significantly rescued by cycloleucine. (A) Western blot was used to detect METTL3 expression in MDA-MB-231 and MDA-MB-468 cells in rescued assay. α-tubulin was used as a loading control. (B,C) Transwell assay was used to detect the migration and invasion ability in MDA-MB-231 and MDA-MB-468 cells with METTL3 transient overexpression and METTL3 overexpression rescued with cycloleucine (left panels). Relative fold change was shown as the proportion of the number of control cells transfected with pcDNA3.1-FLAG (right panels). Original magnification, 100×. (D) Adhesion assay was used to detect the adhesion ability of MDA-MB-231 and MDA-MB-468 cells with METTL3 transient overexpression (left panels). Relative fold change was shown as the proportion of the number of control cells transfected with pcDNA3.1-FLAG (right panels). Original magnification, 100×. *P < 0.05, **P < 0.01. Error bars represent the mean ± SD of three independent experiments.