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. 2020 Jul 14;10:1126. doi: 10.3389/fonc.2020.01126

Figure 4.

Figure 4

COL3A1 was identified as a potential target of METTL3 in TNBC. (A) Flowchart for screening potential target genes. (B,C) qRT-PCR was used to detect COL3A1 and MYH11 expression in MDA-MB-231 and MDA-MB-468 cells transfected with the si-NC or the si-METTL3. 18S was used as an internal control. (D) Western blot was used to detect COL3A1 expression in MDA-MB-231 and MDA-MB-468 cells transfected with the si-NC or the si-METTL3. α-tubulin was used as a loading control (left panel). The blots were scanned and the abundance assessed quantitatively using ImageJ (right panel). *P < 0.05, **P < 0.01. Error bars represent the mean ± SD of three independent experiments.