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. 2020 Jul 14;10:1126. doi: 10.3389/fonc.2020.01126

Figure 6.

Figure 6

COL3A1 promoted the metastatic ability of TNBC cells. (A) Western blot was used to detect COL3A1 expression in MDA-MB-231 and MDA-MB-468 cells transfected with the si-NC or the si-COL3A1. α-tubulin was used as a loading control. (B) Kaplan–Meier analysis for the DMFS of COL3A1 in TNBC patients using KM-plotter online database. (C,D) The migration and invasion of MDA-MB-231 and MDA-MB-468 with transient COL3A1-KD was detected by transwell assays (left panels). Relative fold change was shown as the proportion of the number of control cells transfected with si-NC (right panels). Original magnification, 100×. (E) The adhesion ability of MDA-MB-231 and MDA-MB-468 after transient COL3A1-KD was evaluated by adhesion assay (left panels). Relative fold change was shown as the proportion of the number of control cells transfected with si-NC (right panels). Original magnification, 100×. *P < 0.05, **P < 0.01. Error bars represent the mean ± SD of three independent experiments.