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. 2020 Jul 14;7:126. doi: 10.3389/fmolb.2020.00126

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Copyright © 2020 Chen, Chen, Zhao, Weng, Song, Fang, Wu, Wang, Zhang, Yang, Wang, Xu and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ZFP36 regulates cell growth and cell migration and invasion. (A) qRT-PCR analysis of ZFP36 expression in the hepatocellular carcinoma cell lines HepG2, SNU475, Hep3B, and Huh7 and the normal liver cell line LO2. (B) Knockdown of ZFP36 (ZFP36 shRNA1#−3#) in HepG2 cells and qRT-PCR assessment of ZFP36 expression. HepG2 cells were transfected with ZFP36-shRNA1# or shRNA2# and Huh7 cells were transfected with ZFP36-OE for ZFP36 overexpression. (C) Overexpression of ZFP36 in Huh7 cells and qRT-PCR assessment of ZFP36 expression. (D,E) Cell growth was determined by the CCK-8 assay. (F,G) cell migration and invasion were detected by Transwell assay. *p < 0.05, **p < 0.01, ***p < 0.001.