Figure 4.

Overexpression of ZFP36 increases sensitivity to 5-Fu treatment in vitro. (A,B) Huh7 and HepG2 cells were treated with 0, 5, 10, 25, 50, 100, 200, and 400 μg/ml 5-Fu for 7 days, to determine the half maximal inhibitory concentration (IC50). The IC50 of Huh7 cells is 135 μg/ml and the IC50 of HepG2 cells is 110 μg/ml. (C,D) A colony formation assay of Huh7 cells transfected with ZFP36 and HepG2 cells transduced with ZFP36 shRNA1# or 2#. Cells were grown for 14 days with or without 135 μg/ml 5-Fu for Huh7 cells or 110 μg/ml 5-Fu for HepG2 cells and stained with crystal violet. The colonies were counted and captured. *p < 0.05, **p < 0.01, ***p < 0.001.