Figure 5.

ZFP36 increases the curative effect of 5-Fu on tumor growth in nude mice. (A) The tumor growth curve of ZFP36 overexpressed in Huh7 cells was compared with vector-expressing cells with or without 5-Fu treatment. HepG2 cells (2 × 10⁶) were infected with negative control (NC) or ZFP36-OE. Infected cells were subcutaneously injected into the flanks of 6-week-old male nude mice to induce tumor. Mice were sacrificed 27 days after injection. Photos were captured, and tumor growth was assessed. (B,C) Average tumor weight was measured and growth curves were generated. (D) Ki67, ZFP36, and PRC1 expression were analyzed by immunohistochemistry (IHC). *p < 0.05, **p < 0.01.