Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 14;7:126. doi: 10.3389/fmolb.2020.00126

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Chen, Chen, Zhao, Weng, Song, Fang, Wu, Wang, Zhang, Yang, Wang, Xu and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

ZFP36 binds to the PRC1 3′UTR. (A) Schematic diagram of the 3′ UTR sequence of PRC1. CR, coding region; UTR, untranslated region, and ARE elements. (B) A luciferase reporter plasmid containing wild-type or mutant PRC1 was co-transfected into 293 cells with pcDNNA3.1-ZFP36 or a negative control (NC). Luciferase activity was determined at 48 h after transfection using the dual-luciferase assay and shown as the relative luciferase activity normalized to renilla activity. (C,D) Association of endogenous ZFP36 with endogenous PRC1 mRNA. After IP of RNA–protein complexes from cell lysates using either anti-ZFP36 antibody or control IgG, RNA was isolated and used in RT-PCR reactions. PRC1 levels of mRNAs in ZFP36 IP or IgG IP materials as measured by qPCR in Huh7 and HepG2 cells. (E,F) Knockdown of ZFP36 (ZFP36 shRNA1#−3#) in HepG2 cells and the overexpression of ZFP36 in Huh7 cells, the protein expression of ZFP36 and PRC1 were detected by western blot analysis. **p < 0.01.