Figure 8.

Overexpression of ZFP36 decreases cell migration and invasion and increases sensitivity to 5-Fu treatment by the downregulation of PRC1 expression. (A,B) Huh7 cells were transfected with negative control (NC), ZFP36-OE alone, or co-transfected with ZFP36-OE and PRC1-OE; HepG2 cells were transfected with scr, ZFP36-shRNA2#, or co-transfected with PRC1-shRNA1# or 2# and ZFP36-shRNA2#. Migration and invasion were detected by Transwell assay. (C,D) Huh7 cells were transfected with NC, ZFP36-OE alone, or co-transfected with ZFP36-OE and PRC1-OE; HepG2 cells were transfected with scr, ZFP36-shRNA2#, or co-transfected with PRC1-shRNA1# or 2# with ZFP36-shRNA2#; Cells were grown for 14 days with 135 μg/ml 5-Fu for Huh7 cells or 110 μg/ml 5-Fu for HepG2 cells and stained with crystal violet. The colonies were counted and captured. *p < 0.05, **p < 0.01.