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. 2020 Jul 14;11:1516. doi: 10.3389/fmicb.2020.01516

FIGURE 2.

FIGURE 2

Salmonella enterica persistence in the apoplast of lettuce leaves. Bacterial population size was estimated at 2 h post inoculation (HPI), and at 1, 10, and 20 days after vacuum inoculation (DAI) with the strain 14028s of S. enterica subsp. enterica serovar Typhimurium at a concentration of (A) 3.5 Log CFU ml–1, (B) 5.4 Log CFU ml–1, or (C) 7.7 Log CFU ml–1. Results are shown as the average of three biological replicates and two technical replicates (n = 6 ± SE). Statistical difference among means was detected with ANOVA followed by the Scoot–Knott test (α = 0.05). Different letters above the bars indicate significant statistical differences among cultivars across all time points. CFU = colony forming unit. g, grams. (D) Representative pictures of lettuce leaves at 20 DAI with each one of the bacterial inoculum dose used (3, 5, or 7 Log CFU ml–1) or with the mock control.