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. 2020 Jul 14;8:581. doi: 10.3389/fcell.2020.00581

TABLE 1.

Primers for PCR analysis of TWNK genomic.

Gene Primer sequence (5′–3′) Size (bp) Temperature (°C)
TWNK-exon 1 F-GGGAGGGAGAAGGGAGAGCAT 1,594 56
R-GAAATGAGTGCAGTGGCGACGGT
TWNK-intron 1,2 F-CCATCGTCTCCTTCCGACAG 1,021 58
R-GTTCCTACACCAGCCAGGAC
TWNK-exon 2,3 F-TCGCCACTGCACTCATTTCC 1,271 64
R-CAACTCATGCCGTGTCCCAC
TWNK-intron 3,4 F-TGTTCTCCTTCCGTGTGCTG 1,456 58
R-CCTCTCCCACTCCTCTCACA
TWNK-exon 4,5 F-GCCACATCACGCTGGTCATC 1,001 60
R-AAACAGGATGGGGCCAAAAG